全文获取类型
收费全文 | 847篇 |
免费 | 64篇 |
国内免费 | 16篇 |
专业分类
林业 | 8篇 |
农学 | 10篇 |
基础科学 | 2篇 |
2篇 | |
综合类 | 161篇 |
农作物 | 21篇 |
水产渔业 | 16篇 |
畜牧兽医 | 554篇 |
园艺 | 146篇 |
植物保护 | 7篇 |
出版年
2023年 | 7篇 |
2022年 | 6篇 |
2021年 | 36篇 |
2020年 | 26篇 |
2019年 | 29篇 |
2018年 | 30篇 |
2017年 | 27篇 |
2016年 | 39篇 |
2015年 | 50篇 |
2014年 | 51篇 |
2013年 | 60篇 |
2012年 | 55篇 |
2011年 | 60篇 |
2010年 | 56篇 |
2009年 | 32篇 |
2008年 | 39篇 |
2007年 | 57篇 |
2006年 | 42篇 |
2005年 | 30篇 |
2004年 | 42篇 |
2003年 | 25篇 |
2002年 | 22篇 |
2001年 | 21篇 |
2000年 | 19篇 |
1999年 | 11篇 |
1998年 | 9篇 |
1997年 | 7篇 |
1996年 | 3篇 |
1995年 | 6篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 6篇 |
1991年 | 4篇 |
1989年 | 3篇 |
1987年 | 4篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1980年 | 2篇 |
1975年 | 1篇 |
排序方式: 共有927条查询结果,搜索用时 500 毫秒
1.
The absence or presence of metastases on thoracic radiographs in 55 female dogs with mammary gland tumors was assessed by comparing the results of a single radiographic examination, using dorsoventral and left lateral views, with clinical and histologic follow-up data. Radiographic abnormalities found in dogs with thoracic metastases were classified as well-defined pulmonary nodules, ill-defined pulmonary nodules, and involvement of pleural effusion. No obvious difference in the effect on the right or left lung lobes was found. The sensitivity, specificity and accuracy for the detection of thoracic metastases were 65%, 97%, and 87%, respectively. 相似文献
2.
Donald E. Thrall DVM PHD Margaret C. McEntee DVM† Carol Novotney DVM† Marlene L. Hauck DVM† Rodney L. Page DVM MS† 《Veterinary radiology & ultrasound》1993,34(4):295-300
Eighteen dogs with malignant nasal cavity tumors were treated with radiation therapy, including a boost technique. Three 3:0 Gy boost doses were added to a treatment protocol consisting of sixteen 3.0 Gy daily fractions, bringing the total dose to 57 Gy. This boost technique was implemented without an associated increase in overall treatment time by giving the boost doses on a twice-a-day basis. Boost doses were given during the first half of the radiation therapy period. The treatment was completed as planned in 16 of the 18 dogs; two dogs received lower doses (51 and 54 Gy). Median survival was 177 days, poorer than in some other reported studies of nasal tumor irradiation. Acute effects were unacceptable, with 11 of the 18 dogs developing severe mucositis, desquamation, edema, swelling, and pruritus. The extensive nature of the acute reactions compromised assessment of the effect of the increased radiation dose on the tumor. Although there is justification for assessing more aggressive radiation protocols in canine nasal tumor patients, total doses approximating 60 Gy can not be given as described because of the inability of acutely responding normal tissues to compensate. 相似文献
3.
用免疫组化A B C法对60例甲状腺病理标本进行了甲状腺球蛋白检查,结果表明源于甲状腺滤泡上皮细胞的肿瘤,均呈阳性或弱阳性反应。大部分甲状腺癌呈弱阳性反应。作者认为甲状腺球蛋白可作为鉴别甲状腺滤泡上皮细胞源性与非滤泡上皮细胞源性的良好标记。 相似文献
4.
David Lipsitz DVM Robin E. Levitski DVM Wayne L. Berry BVSc MMedVet 《Veterinary radiology & ultrasound》2001,42(1):14-19
Three dogs with multilobular osteochondrosarcoma of the skull were evaluated using magnetic resonance (MR) imaging. Spin echo T1, T2, proton weighted and post contrast T1W images were obtained with a 1.5 Tesla magnet. The MR imaging findings were similar in all three dogs with mixed signal intensities in the T1W, T2W and proton weighted images and fairly large areas of contrast enhancement in the post contrast T1W images. The extent of brain and soft tissue involvement were well delineated and provided useful information concerning surgical planning. MR imaging provided a useful method of evaluating dogs with skull tumors. 相似文献
5.
目的:总结桥小脑角区巨大肿瘤的诊治经验。方法:对我科73例桥小脑角区巨大肿瘤的发病率、临床和影像学表现、定位与定性诊断、手术治疗及随访期心理治疗等作分析。结果:本组73例桥小脑角巨大肿瘤(均≥4cm)中,属神经鞘瘤50例(68.5%)、脑膜瘤19例(26.0%)与胆脂瘤4例(5.5%)。临床特点:73例均有所神经、小脑损害的症状与体征及颅内高压征。73例中手术全切除者60例、次全切除l3例(其中10例残留肿瘤,结合枷码刀治疗)。全切除组中2例死于肺部感染,次全切除组中3例放弃治疗,余者随访3—5a,无复发。在颅神经损伤所致并发症的56例中,出现心身疾病须心理治疗者40例(71.4%)。结论:临床特点结合影像学检查可作出早期定位与定性诊断.并有助于手术设计。提高疗效的关键是早诊断、早治疗以及娴熟的显微操作技术。手术结合枷码刀以及心理治疗可改善预后、提高生存质量。 相似文献
6.
7.
AIM:To explore the mechanism of neuronal injury and repair by investigating the expression of caspase-3 and apurinic/apyrimidinic endonuclease (APE/Ref-1) after focal cerebral ischemia. METHODS:A model of middle cerebral artery occlusion in rats was performed. The expression of caspase-3P20 and APE/Ref-1 was examined by immunohistochemistry staining, TUNEL was applied to detected DNA damage, and double labeling with TUNEL and APE/Ref-1 was used to determine the relationship between APE/Ref-1 and DNA damage. RESULTS:The active subunit P20 of caspase-3 was predominantly expressed within ischemic penumbra. The peak time of caspase-3P20 positive cells preceded the appearance of TUNEL. With aggravation of cerebral ischemia, APE/Ref-1 immunoreactive cells in penumbra were significantly decreased. CONCLUSION:The activation of caspase enzymatic cascade following cerebral ischemia leads to degradation in DNA, meanwhile, decrease in DNA repair molecules or the failure of DNA repair may deteriorate the course. 相似文献
8.
AIM:To explore the effect of brain ischemia injury on cell proliferation and nestin expression in cortex and subependymal zone (SEZ).METHODS:Using a local brain ischemia model(MCAO), BrdU positive cells of cortex and subependymal zone (SEZ), also nestin positive cells, were observed by immunohistochemistry.RESULTS:BrdU and nestin positive cells in SEZ of MCAO rats were obviously increased. In cortex, only nestin positive cells were observed.CONCLUSION:Neural stem cells in SEZ and cortex were activated after brain ischemia, it may be related with neural recovery after brain ischemia injury. 相似文献
9.
AIM:To explore transdifferentiation potential of Sca-1+ cells from murine fetal liver. METHODS:2×103 of Sca-1+ cells from male murine fetal liver were transfused into female mouse irradiated lethally with γ ray from 60 Co source (10 Gy) via tail vein. Two months later, FISH and immunohistochemistry were used to detect the situation for transdifferentiating of the donor cells (male cells) in tissues of female recipient mouse. RESULTS:The renal tubular epitheliocyte-like and neurocyte-like cells with Y chromosome were found on the sections of renal and brain tissues from female recipient mice. These cells have phenotype characteristics of RCA+/CD45-F4/80- and NueN+/CD45-F4/80-, respectively. CONCLUSION:The evidence is provided for Sca-1+ cells from murine fetal liver to transdifferentiate into both renal and brain tissue cells. 相似文献
10.
AIM:To explore the expressive profile of nestin protein in the focal ischemic brain and to study the recovery mechanism of brain focal infarct.METHODS:Cellular morphology,time-course and distribution pattern of nestin positive response were immunohistochemically examined in different brain regions of 36 adult male SD rats. RESULTS:Nestin positive response of different brain regions in sham operated rats was present in small- and micro-vasculartures and the third ventricle bottom and ependyma. A large number of nestin positive cells were detected in ischemic brain, and were more remarkable in the cortical areas of parietal lobe and preoptic area as well as ischemic caudoputamen. Stellate nestin positive cells were located in the deep layer of ischemic cortex, but fibrillary cells were located in the shallow layer. Nestin positive cells in the ischemic caudoputamen showed the same changes of morphology as those cells in the deep layer of ischemic cortex. Morphological and number alterations of nestin positive cells were the most remarkable at 1 weeks post-ischemia, which showed more hypertrophy and proliferation in morphology, and a marked increase in number was present in the ischemic cerebral cortex and the ischemic caudoputamen. These alterations of nestin positive cells persisted up to 6 weeks post-ischemia, and then, the nestin positive response in the ischemic brain decreased gradually.CONCLUSION:Focal cerebral ischemia induces nestin re-expression on reactive astrocytes, which may be very important to the self-recovery of cerebral infarct. 相似文献